Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice
نویسندگان
چکیده
منابع مشابه
Biology of germinal centers in lymphoid tissue.
Germinal centers in lymphoid tissue are the sites of generation of memory B cells undergoing isotype switching and somatic mutation in their Ig genes. Their formation cannot be induced by stimuli other than immunogenic ones. It seems likely that in the function and possibly also in the formation of germinal centers, one important factor is the localization of immune complexes with fixed complem...
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IsHII, Y., MORI, M. and FUJIMOTO, T. Immunofluorescence Study of Germinal Centers in Mouse Lymph Nodes during the Secondary Immune Response. Tohoku J. exp. Med., 1970, 102(2), 143-157 An immunofluorescence study carried out on the relationship between the mode of localization of antigen and that of specific antibody and ƒÁ-globulin in the germinal centers of mouse popliteal lymph nodes during t...
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Recent years have seen a powerful revival of fluorescence microscopy techniques, both to observe live cells and fixed objects. The limits of sensitivity, simultaneous detection of multiple chromophores, and spatial resolution have all been pushed to the extreme. Therefore, it is essential to improve in parallel the quality of the structural and antigenic preservation during fixation and immunos...
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The fate, proliferation, and developmental potentialities of cell suspensions made from white pulp containing large germinal centers have been studied in the mouse by transfer of cells labeled with thymidine-(3)H to lethally irradiated, syngeneic recipients. Radioautographic analyses were made using both smears and sections of a variety of tissues. Thymidine-(3)H-labeling patterns of white pulp...
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ژورنال
عنوان ژورنال: STAR Protocols
سال: 2021
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2021.100499